HLVd Research
Clean from the start
Hop latent viroid (HLVd) spreads quietly from plant to plant in cannabis nurseries. We take extra steps to keep it out of ours, and we test to make sure.
- Paired base
- Unpaired base, in a loop
- Base pair
01What HLVd is
Hop latent viroid is not a virus. It is a viroid: a tiny circle of RNA, 256 bases long, with no protein coat. Infected plants often look healthy, but over time it can stunt growth, weaken vigor and leave smaller, less potent flowers. It spreads through cuttings taken from an infected plant and on tools that touch one, and no spray or chemical cures an infected plant.
02How we keep it out
- Tissue culture
- Our mother plants start from the plant's growing tip, cultured in our sterile culture room. Tissue culture lets us rebuild clean stock and keep it in sterile conditions, away from the plants in the nursery.
- Clean tools and surfaces
- Tools and work surfaces are cleaned with fresh bleach solution, which inactivates the viroid where alcohol alone does not.
03Routine testing
We routinely send plant material for HLVd testing by RT-qPCR. Each plant is sampled with its own sterile, single-use tools and fresh gloves, so nothing carries from one plant to the next. Clones are tested from three weeks old, from root, stem and leaf, and a clone counts as positive if any one of them is.
04Research
We take part in HLVd research at the analytical laboratory, contributing plant material from our mother stock to the development of molecular testing for cannabis root, stem and leaf tissue.
Stem Analytical
Are you 21 or older?
You must be 21 or older to visit this site.